Superresolution Microscopy of Sphingolipids

Jan Schlegel; Markus Sauer

doi:10.1007/978-1-0716-0814-2_17

Superresolution Microscopy of Sphingolipids

Methods Mol Biol. 2021:2187:303-311. doi: 10.1007/978-1-0716-0814-2_17.

Authors

Jan Schlegel¹, Markus Sauer²

Affiliations

¹ Department of Biotechnology and Biophysics, Biocenter, University of Wuerzburg, Wuerzburg, Germany.
² Department of Biotechnology and Biophysics, Biocenter, University of Wuerzburg, Wuerzburg, Germany. m.sauer@uni-wuerzburg.de.

PMID: 32770514
DOI: 10.1007/978-1-0716-0814-2_17

Abstract

This chapter provides a step-by-step protocol to label and visualize sphingolipids by superresolution microscopy with a special focus on single-molecule localization microscopy by dSTORM. We provide information on custom fluorophore conjugation to raft-associated toxins and antibodies, and a labeling protocol for appropriate sample treatment.

Keywords: Sphingolipids; Superresolution microscopy; dSTORM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Microscopy, Fluorescence / methods*
Single Molecule Imaging / methods*
Sphingolipids / metabolism*

Substances

Sphingolipids