Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples

Ying Xu; Biao Ma; Erjing Chen; Xiaoping Yu; Zihong Ye; Chuanxin Sun; Mingzhou Zhang

doi:10.1016/j.foodchem.2020.127713

Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples

Food Chem. 2021 Jan 30:336:127713. doi: 10.1016/j.foodchem.2020.127713. Epub 2020 Jul 31.

Authors

Ying Xu¹, Biao Ma², Erjing Chen³, Xiaoping Yu⁴, Zihong Ye⁵, Chuanxin Sun⁶, Mingzhou Zhang⁷

Affiliations

¹ Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: s1809071028@cjlu.edu.cn.
² Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: 16a0701109@cjlu.edu.cn.
³ Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: s1709071002@cjlu.edu.cn.
⁴ Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: yxp@cjlu.edu.cn.
⁵ Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: zhye@cjlu.edu.cn.
⁶ Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, Swedish University of Agricultural Science (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden. Electronic address: Chuanxin.Sun@slu.se.
⁷ Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China. Electronic address: zmzcjlu@cjlu.edu.cn.

PMID: 32768909
DOI: 10.1016/j.foodchem.2020.127713

Abstract

The presence of multiple mycotoxins in the agricultural products poses a serious threat to the health of humans and animals. Citrinin (CIT) causes slow growth in animals and damages the kidney function. Zearalenone (ZEN) causes chronic poisoning, abnormal functioning and even death in animals. Herein, a dual fluorescent immunochromatographic assay (DF-ICA) based on europium nanoparticles (EuNPs) was developed for the simultaneous detection of CIT and ZEN in the corn samples. After optimization, the limits of detection (LODs), IC₅₀ and average recoveries for the simultaneous determination of CIT and ZEN were 0.06 and 0.11 ng/mL, 0.35 and 0.76 ng/mL, from 86.3% to 111.6% and from 86.6% to 114.4%, respectively. Moreover, the DF-ICA was validated by high performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained. In brief, this work demonstrates the feasibility of DF-ICA for simultaneous monitoring of CIT and ZEN in the corn samples.

Keywords: Citrinin; Corn; Europium nanoparticles; Fluorescent immunochromatographic assay; Simultaneous detection; Zearalenone.

MeSH terms

Animals
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology
Chromatography, High Pressure Liquid
Citrinin / analysis*
Citrinin / immunology
Europium / chemistry
Fluorescence
Food Analysis / instrumentation
Food Analysis / methods
Food Contamination / analysis*
Humans
Immunoassay / methods*
Limit of Detection
Metal Nanoparticles / chemistry
Reproducibility of Results
Sensitivity and Specificity
Zea mays / chemistry*
Zearalenone / analysis*
Zearalenone / immunology

Substances

Antibodies, Monoclonal
Citrinin
Europium
Zearalenone