A robust spectral angle index for remotely assessing soybean canopy chlorophyll content in different growing stages

Jibo Yue; Haikuan Feng; Qingjiu Tian; Chengquan Zhou

doi:10.1186/s13007-020-00643-z

A robust spectral angle index for remotely assessing soybean canopy chlorophyll content in different growing stages

Plant Methods. 2020 Jul 31:16:104. doi: 10.1186/s13007-020-00643-z. eCollection 2020.

Authors

Jibo Yue^{1

2}, Haikuan Feng¹, Qingjiu Tian², Chengquan Zhou^{1

3}

Affiliations

¹ Key Laboratory of Quantitative Remote Sensing in Agriculture of Ministry of Agriculture, Beijing Research Center for Information Technology in Agriculture, Beijing, 100097 China.
² International Institute for Earth System Science, Nanjing University, Nanjing, 210023 China.
³ Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences (ZAAS), Hangzhou, 310000 China.

Abstract

Background: Timely and accurate estimates of canopy chlorophyll (Chl) a and b content are crucial for crop growth monitoring and agricultural management. Crop canopy reflectance depends on many factors, which can be divided into the following categories: (i) leaf effects (e.g., leaf pigments), (ii) canopy effects (e.g., Leaf Area Index [LAI]), and (iii) soil background reflectance (e.g., soil reflectance). The estimation of leaf variables, such as Chl contents, from reflectance at the canopy scale is usually less accurate than that at the leaf scale. In this study, we propose a Visible and Near-infrared (NIR) Angle Index (VNAI) to estimate the Chl content of soybean canopy, and soybean canopy Chl maps are produced using visible and NIR unmanned aerial vehicle (UAV) remote sensing images. The VNAI is insensitive to LAI and can be used for the multi-stage estimation of crop canopy Chl content.

Results: Eleven previously used vegetation indices (VIs) (e.g., Pigment-specific Normalized Difference Index) were selected for performance comparison. The results showed that (i) most previously used Chl VIs were significantly correlated with LAI, and the proposed VNAI was more sensitive to Chl content than LAI; (ii) the VNAI-based estimates of Chl content were more accurate than those based on the other investigated VIs using (1) simulated, (2) real (field), and (3) real (UAV) datasets.

Conclusions: Most previously used Chl VIs were significantly correlated with LAI whereas the proposed VNAI was more sensitive to Chl content than to LAI, indicating that the VNAI may be more strongly correlated with Chl content than these previously used VIs. Multi-stage estimations of the Chl content of cropland obtained using the VNAI and broadband remote sensing images may help to obtain Chl maps with high temporal and spatial resolution.

Keywords: Angle index; Soybean; Spectral vegetation indices; UAV remote sensing.