A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

Dae-Kyum Kim; Jennifer J Knapp; Da Kuang; Aditya Chawla; Patricia Cassonnet; Hunsang Lee; Dayag Sheykhkarimli; Payman Samavarchi-Tehrani; Hala Abdouni; Ashyad Rayhan; Roujia Li; Oxana Pogoutse; Étienne Coyaud; Sylvie van der Werf; Caroline Demeret; Anne-Claude Gingras; Mikko Taipale; Brian Raught; Yves Jacob; Frederick P Roth

doi:10.1534/g3.120.401554

A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

G3 (Bethesda). 2020 Sep 2;10(9):3399-3402. doi: 10.1534/g3.120.401554.

Authors

Dae-Kyum Kim^{1

2

3}, Jennifer J Knapp^{1

2

3}, Da Kuang^{1

2

3}, Aditya Chawla^{1

2

3}, Patricia Cassonnet^{4

5

6}, Hunsang Lee^{1

2}, Dayag Sheykhkarimli^{1

2

3}, Payman Samavarchi-Tehrani³, Hala Abdouni³, Ashyad Rayhan^{1

2

3}, Roujia Li^{1

2

3}, Oxana Pogoutse^{1

2

3}, Étienne Coyaud⁷, Sylvie van der Werf^{4

5

6}, Caroline Demeret^{4

5

6}, Anne-Claude Gingras^{2

3}, Mikko Taipale^{1

2

8}, Brian Raught⁹, Yves Jacob^{10

5

6}, Frederick P Roth^{11

2

3

12}

Affiliations

¹ Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
² Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
³ Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
⁴ Unité de Génétique Moléculaire des Virus à ARN, Département Virologie, Institut Pasteur, Paris, France.
⁵ UMR3569, Centre National de la Recherche Scientifique, Paris, France.
⁶ Université de Paris, Paris, France.
⁷ Univ. Lille, Inserm, CHU Lille, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, F-59000 Lille, France.
⁸ Molecular Architecture of Life Program, Canadian Institute for Advanced Research, Toronto, Ontario, Canada.
⁹ Department of Medical Biophysics, Princess Margaret Cancer Centre, University of Toronto, Toronto, Ontario, Canada.
¹⁰ Unité de Génétique Moléculaire des Virus à ARN, Département Virologie, Institut Pasteur, Paris, France yves.jacob@pasteur.fr fritz.roth@utoronto.ca.
¹¹ Donnelly Centre, University of Toronto, Toronto, Ontario, Canada yves.jacob@pasteur.fr fritz.roth@utoronto.ca.
¹² Department of Computer Science, University of Toronto, Toronto, Ontario, Canada.

Abstract

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Keywords: Gateway-compatible; SARS-CoV-2; TEV (tobacco etch virus) sequence; coding sequence collection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Betacoronavirus / genetics*
Betacoronavirus / isolation & purification
COVID-19
Cloning, Molecular
Coronavirus Infections / pathology
Coronavirus Infections / virology
Escherichia coli / metabolism
Humans
Open Reading Frames / genetics*
Pandemics
Plasmids / genetics
Plasmids / metabolism
Pneumonia, Viral / pathology
Pneumonia, Viral / virology
Potyvirus / genetics
SARS-CoV-2

Supplementary concepts

Tobacco etch virus

Associated data

figshare/10.25387/g3.12725096

Grants and funding

Canadian Institutes for Health Research/International