Terabase Metagenome Sequencing of Grassland Soil Microbiomes

William C Nelson; Lindsey N Anderson; Ruonan Wu; Jason E McDermott; Sheryl L Bell; Ari Jumpponen; Sarah J Fansler; Kimberly J Tyrrell; Yuliya Farris; Kirsten S Hofmockel; Janet K Jansson

doi:10.1128/MRA.00718-20

Terabase Metagenome Sequencing of Grassland Soil Microbiomes

Microbiol Resour Announc. 2020 Aug 6;9(32):e00718-20. doi: 10.1128/MRA.00718-20.

Authors

Affiliations

¹ Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA william.nelson@pnnl.gov janet.jansson@pnnl.gov.
² Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA.
³ Division of Biology, Kansas State University, Manhattan, Kansas, USA.
⁴ Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA.

^# Contributed equally.

Abstract

To enable an in-depth survey of the metabolic potential of complex soil microbiomes, we performed ultra-deep metagenome sequencing, collecting >1 Tb of sequence data from three grassland soils representing different precipitation regimes.