Application of cold atmospheric pressure plasmas for high-throughput production of safe-to-consume beetroot juice with improved nutritional quality

Food Chem. 2021 Jan 30:336:127635. doi: 10.1016/j.foodchem.2020.127635. Epub 2020 Jul 25.

Abstract

A one-step, highly-efficiency, and low-cost cold atmospheric pressure plasma (CAPP)-based method for obtaining safe-to-consume beetroot juice (BRJ) with enhanced nutritional quality is presented. Three reaction-discharge systems with different CAPPs were studied to check how the composition and physicochemical properties changed during CAPP treatment of BRJ. To identify reactive species occur in gas phase of applied CAPP for BRJ treatment, optical emission spectrometry was used. Finally, the cytotoxicity of so-obtained BRJ to human epithelial colorectal adenocarcinoma (Caco-2) and human non-malignant intestine microvascular endothelial cells (HIMEC) was assessed. Based on the performed analyses it was found that controlled CAPP treatment of BRJ changes the fraction pattern of elements in addition to increase the content of phenolic compound presents in BRJ. Furthermore, the defined CAPP treatment of BRJ inhibits proliferation of Caco-2 cell lines, exhibiting non-cytotoxic effect for HIMEC non-malignant endothelial cells. As a result, safe-to-consume BRJ of improved nutritional quality was produced.

Keywords: Atmospheric pressure glow discharges; Beverages; Cytotoxicity; Metals.

MeSH terms

  • Antioxidants / chemistry
  • Atmospheric Pressure
  • Beta vulgaris / chemistry*
  • Caco-2 Cells
  • Carbohydrates / analysis
  • Endothelial Cells / drug effects
  • Food-Processing Industry / methods*
  • Fruit and Vegetable Juices* / analysis
  • Fruit and Vegetable Juices* / toxicity
  • Humans
  • Metals / analysis
  • Nutritive Value
  • Phenols / analysis
  • Plasma Gases*
  • Toxicity Tests

Substances

  • Antioxidants
  • Carbohydrates
  • Metals
  • Phenols
  • Plasma Gases