Magnetic targeting of super-paramagnetic iron oxide nanoparticle labeled myogenic-induced adipose-derived stem cells in a rat model of stress urinary incontinence

Ying Wang; Shukui Zhou; Ranxing Yang; Mahbubur Rahman; Russel Clive Sequeira; Nailong Cao; Yang Zhang; Weixin Zhao; Qiang Fu

doi:10.1016/j.nano.2020.102281

Magnetic targeting of super-paramagnetic iron oxide nanoparticle labeled myogenic-induced adipose-derived stem cells in a rat model of stress urinary incontinence

Nanomedicine. 2020 Nov:30:102281. doi: 10.1016/j.nano.2020.102281. Epub 2020 Aug 5.

Authors

Ying Wang¹, Shukui Zhou², Ranxing Yang³, Mahbubur Rahman⁴, Russel Clive Sequeira⁵, Nailong Cao¹, Yang Zhang⁶, Weixin Zhao⁷, Qiang Fu⁸

Affiliations

¹ Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Eastern Institute of Urologic Reconstruction, Shanghai Jiao Tong University, Shanghai, China.
² Department of Urology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
³ Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Eastern Institute of Urologic Reconstruction, Shanghai Jiao Tong University, Shanghai, China; Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Winston-Salem, North Carolina, USA.
⁴ Department of General Educational Development, Faculty of Science and Information Technology, Daffodil International University, Dhaka, Bangladesh; Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.
⁵ Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Winston-Salem, North Carolina, USA.
⁶ Department of Pharmacy, Taizhou Central Hospital, Zhejiang, China; Institute for Intestinal Diseases, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address: zy_benjamin@163.com.
⁷ Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Winston-Salem, North Carolina, USA. Electronic address: wezhao@wakehealth.edu.
⁸ Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Eastern Institute of Urologic Reconstruction, Shanghai Jiao Tong University, Shanghai, China. Electronic address: jamesqfu@aliyun.com.

PMID: 32763385
DOI: 10.1016/j.nano.2020.102281

Abstract

Cell-based injectable therapy utilizing stem cells is a promising approach for the treatment of stress urinary incontinence (SUI). Applying a magnetically controlled cell delivery approach has enormous potential to enhance cell retention capability within the specified site. To assess the therapeutic efficacy of cellular magnetic targeting, we applied an external magnetic force to target an adipose-derived stem cell based therapy in a rat model of SUI. The results revealed that magnetic attraction of transplanted cells under the magnetic field was generated by cell uptake of superparamagnetic iron oxide nanoparticles in vitro. More importantly, magnetic targeting improved the retention rate of transplanted cells and facilitated the restoration of sphincter structure and function in a rat SUI model according to the results of histological examination and urodynamic testing. Therefore, magnetically guided targeting strategy might be a potential therapy method for treatment of SUI.

Keywords: Adipose derived stem cells; Cell transplantation; Magnetic targeting; Stress urinary incontinence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / cytology*
Animals
Cell Differentiation
Disease Models, Animal
Female
Magnetic Iron Oxide Nanoparticles / chemistry*
Microscopy, Electron, Transmission
Rats
Rats, Sprague-Dawley
Stem Cell Transplantation / methods*
Stem Cells / cytology*
Urinary Incontinence, Stress / therapy*