Aerobic denitrifying bacterial communities drive nitrate removal: Performance, metabolic activity, dynamics and interactions of core species

Sulin Li; Haihan Zhang; Tinglin Huang; Ben Ma; Yutian Miao; Yinjie Shi; Lei Xu; Kaiwen Liu; Xin Huang

doi:10.1016/j.biortech.2020.123922

Aerobic denitrifying bacterial communities drive nitrate removal: Performance, metabolic activity, dynamics and interactions of core species

Bioresour Technol. 2020 Nov:316:123922. doi: 10.1016/j.biortech.2020.123922. Epub 2020 Jul 30.

Authors

Sulin Li¹, Haihan Zhang², Tinglin Huang¹, Ben Ma¹, Yutian Miao¹, Yinjie Shi¹, Lei Xu¹, Kaiwen Liu¹, Xin Huang¹

Affiliations

¹ Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
² Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: zhanghaihan@xauat.edu.cn.

PMID: 32758920
DOI: 10.1016/j.biortech.2020.123922

Abstract

Three novel mix-cultured aerobic denitrifying bacteria (Mix-CADB) consortia named D14, X21, and CL exhibited excellent total organic carbon (TOC) removal and aerobic denitrification capacities. The TOC and nitrate removal efficiencies were higher than 93.00% and 98.00%. The results of Biolog demonstrated that three communities displayed high carbon metabolic activity. nirS gene sequencing and ecological network model revealed that Pseudomonas stutzeri, Paracoccus sp., and Paracoccus denitrificans dominated in the D14, X21, and CL communities. The dynamics and co-existence of core species in communities drove the nutrient removal. Response surface methodology showed the predicted total nitrogen removal efficiency reached 99.43% for D14 community. The three Mix-CADB consortia have great potential for nitrogen-polluted aquatic water treatment because of their strong adaptability and removal performance. These results will provide new understanding of co-existence, interaction and dynamics of Mix-CADB consortia for nitrogen removal in nitrogen-polluted aquatic ecosystems.

Keywords: Aerobic denitrification; Co-existence; Dynamics and interactions; nirS gene.

MeSH terms

Aerobiosis
Denitrification*
Ecosystem*
Nitrates
Nitrogen

Substances

Nitrates
Nitrogen