This paper originally proposes a wireless multisensor module with illuminance, temperature, relative humidity (RH) and carbon dioxide (CO2) sensors in an aseptic jar incubator for a solid-state fermentation (SSF) of Cordyceps militaris culture. The light intensity, ambient temperature, RH and CO2 are the critical cultivation factors of C. militaris. First, these sensors are integrated in a multisensor platform which is installed inside a lid and covered with a high-efficiency particulate air (HEPA) membrane of class H14 for sterilization of bacteria and viruses. The observations of sensors are then transmitted by a wireless XBee network where the slave sensor node is fixed at the top of jar lid and the master radio node receives data and uploads to an on-site monitoring node. The acquired information is further transmitted to an iCloud database and displayed in a web-based monitoring system. The results illustrate the proposed wireless multisensor module was validated with sufficient accuracy, reliable confidence and well-tolerance for C. militaris cultivation biotechnology under aseptic conditions.
Keywords: Cordyceps militaris culture; solid-state fermentation (SSF); sterilized jar incubator; wireless multisensor system.