Influence of different ceramic materials and surface treatments on the adhesion of Prevotella intermedia

Abstract

Ceramics are used in oral rehabilitation; however, these materials are prone to formation of biofilms that may cause periodontal diseases. This study aimed to evaluate the influence of distinct surface treatments on ceramic surface roughness and biofilm formation of oral bacteria (Prevotella intermedia). Eighty-four specimens of the following four ceramic systems were produced: LC - leucite-based glass ceramic, LD - lithium disilicate-based glass ceramic, LSZ - glass ceramic based on zirconia-reinforced lithium silicate, and ZR - monolithic zirconia. These were submitted to three different surface treatment protocols: C - control, G - glazing, and GDB - grinding with diamond bur (n = 7). The surface characteristics were assessed using a confocal laser microscope (Ra) and a scanning electron microscope (SEM). Thereafter, the groups were contaminated with a bacterial strain of P. intermedia ATCC 25611. The biofilms formed were quantified by counting the colony forming units (CFUs) and analyzed with scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). Data were analyzed by using a 2-way ANOVA and the Tukey test (ɑ = 0.05). Results showed that greater roughness was associated with GDB (p < 0.05). The same was also true for the ceramic material ZR (p < 0.05). There was a statistical significant difference in the CFU counts between the materials (p < 0.05) that revealed a greater amount of bacterial adhesion in the LC and ZR groups (p > 0.05). Thus, it was suggested that the surface roughness of the ceramic materials favored bacterial adhesion; and thus, finishing of ceramic surfaces with GDB should be avoided.

Keywords: Ceramics. CAD-CAM. Dental biofilm. surface roughness.