PLLA Membrane with Embedded Hydroxyapatite Patterns for Improved Bioactivity and Efficient Delivery of Growth Factor

In-Gu Kang; Jinyoung Kim; Suhyung Park; Hyoun-Ee Kim; Cheol-Min Han

doi:10.1002/mabi.202000136

PLLA Membrane with Embedded Hydroxyapatite Patterns for Improved Bioactivity and Efficient Delivery of Growth Factor

Macromol Biosci. 2020 Oct;20(10):e2000136. doi: 10.1002/mabi.202000136. Epub 2020 Aug 4.

Authors

In-Gu Kang¹, Jinyoung Kim¹, Suhyung Park¹, Hyoun-Ee Kim^{1

2}, Cheol-Min Han³

Affiliations

¹ Department of Materials Science and Engineering, Seoul National University, Seoul, 08826, South Korea.
² Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology, Suwon, 16629, South Korea.
³ Department of Carbon and Nano Materials Engineering, Jeonju University, Jeonju, 55069, South Korea.

PMID: 32748557
DOI: 10.1002/mabi.202000136

Abstract

Poly(l-lactic) acid (PLLA) is widely used in guided bone regeneration membranes due to its mechanical properties and biodegradability. However, the lack of biocompatibility is a serious disadvantage. Herein, the biocompatibility of PLLA is improved by patterning hydroxyapatite (HA) loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) under it. The HA is obtained by preparing a magnesium pattern via photolithography and hydrothermal converting. After loading rhBMP-2, the pattern is transferred to PLLA. The pattern is tightly embedded in the PLLA and retained its original position after mechanical stimuli. Fluorescence images allow to assess the protein adsorption and gradual release in a controlled manner. The amount of released rhBMP-2 is overwhelmingly large when loaded under HA because of its large surface area. Osteogenic differentiation supports the synergistic effect of HA and rhBMP-2 to improve the biocompatibility. Moreover, in vivo experiments demonstrate that the synergistic effect positively affects the healing rate of bone.

Keywords: growth factor delivery; guided bone regeneration membrane; hydroxyapatite; patterning; poly( l-lactic) acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Morphogenetic Protein 2 / pharmacology
Cell Line
Cell Survival / drug effects
Drug Delivery Systems*
Drug Liberation
Humans
Intercellular Signaling Peptides and Proteins / pharmacology*
Male
Membranes, Artificial*
Mice
Polyesters / chemistry*
Rabbits
Recombinant Proteins / pharmacology
Skull / diagnostic imaging
Skull / drug effects
Skull / pathology
Surface Properties
Transforming Growth Factor beta / pharmacology
X-Ray Microtomography

Substances

Bone Morphogenetic Protein 2
Intercellular Signaling Peptides and Proteins
Membranes, Artificial
Polyesters
Recombinant Proteins
Transforming Growth Factor beta
recombinant human bone morphogenetic protein-2
poly(lactide)