A cost-effective portable glucose monitoring system with remote data access based on a novel e-oscilloscope was developed using a glucose biofuel cell and a capacitor circuit interfaced to an ESP8266 microcontroller programmed to convert the charge/discharge rates of the capacitor functioning as a transducer. The capacitor charge/discharge rates were converted into glucose concentration readings that is monitored remotely. The glucose monitoring system comprise a glucose biofuel cell, a charge pump circuit, a capacitor and an ESP microcontroller. The anode was fabricated by modifying a gold microwire with nanoporous colloidal platinum (Au-co-Pt) and the cathode was constructed using a mesh dense network of multiwalled carbon nanotubes modified with bilirubin oxidase, respectively. The glucose monitoring system showed sensitivity of 1.18 Hz/mM · cm2 with a correlation coefficient of 0.9939 with increasing glucose concentration from 1 mM to 25 mM. In addition, the glucose monitoring system exhibited optimal operation at a pH of 7.4 and 37 °C, which is ideal for physiological glucose monitoring.