A new integrated HPTLC - ATR/FTIR approach in marine algae bioprofiling

Snezana Agatonovic-Kustrin; Galina Ramenskaya; Ella Kustrin; Davoud Babazadeh Ortakand; David W Morton

doi:10.1016/j.jpba.2020.113488

A new integrated HPTLC - ATR/FTIR approach in marine algae bioprofiling

J Pharm Biomed Anal. 2020 Sep 10:189:113488. doi: 10.1016/j.jpba.2020.113488. Epub 2020 Jul 22.

Authors

Snezana Agatonovic-Kustrin¹, Galina Ramenskaya², Ella Kustrin³, Davoud Babazadeh Ortakand⁴, David W Morton⁵

Affiliations

¹ Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991, Russia; School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
² Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991, Russia.
³ Department of Creative Arts and English, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
⁴ School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
⁵ Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991, Russia; School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia. Electronic address: d.morton@latrobe.edu.au.

PMID: 32745905
DOI: 10.1016/j.jpba.2020.113488

Abstract

The aim of this study was to evaluate marine algae extracts in terms of their anti-inflammatory activity using a combination of chromatographic separation and chemical detection with subsequent infrared vibrational spectroscopy identification. Extraction parameters, chemical fingerprint, and the activity levels were considered for the method optimization. High-performance thin-layer chromatography (HPTLC) combined with microchemical derivatization, was used to separate and detect bioactive compounds with antioxidant activity and anti-inflammatory activities, and to detect different classes of terpenoids. Infrared attenuated total reflectance (ATR) spectral analysis of the bands with bioactive compounds, identified sulfated polysaccharides to be responsible for the anti-inflammatory activity in extracts of brown algae Carpoglossum confluens and Phyllospora comosa. Steroids as unique antioxidants with significant free radical scavenging activities were observed in extracts of brown algae Cystophora platylobium, Cystophora retorta, Carpoglossum confluens and Phyllospora comosa. HPTLC combined with biochemical assays and FTIR-ATR spectrometry was demonstrated to be a straightforward strategy for bioprofiling marine algae extracts.

Keywords: HPTLC bioprofiling; anti-inflammatory activity; antioxidant steroids; marine algae; sulfated polysaccharides.

MeSH terms

Antioxidants / pharmacology
Chromatography, Thin Layer
Phaeophyceae*
Plant Extracts* / pharmacology
Spectroscopy, Fourier Transform Infrared

Substances

Antioxidants
Plant Extracts