Parallel derivation of X-monosomy induced pluripotent stem cells (iPSCs) with isogenic control iPSCs

Anis Feki; Frédérique Sloan-Béna; Youssef Hibaoui

doi:10.1016/j.scr.2020.101920

Parallel derivation of X-monosomy induced pluripotent stem cells (iPSCs) with isogenic control iPSCs

Stem Cell Res. 2020 Jul 22:47:101920. doi: 10.1016/j.scr.2020.101920. Online ahead of print.

Authors

Anis Feki¹, Frédérique Sloan-Béna², Youssef Hibaoui³

Affiliations

¹ Service de gynécologie obstétrique, HFR Fribourg - Hôpital cantonal, Chemin des Pensionnats 2-6, Case postale, 1708 Fribourg, Switzerland; Stem Cell Research Laboratory, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland. Electronic address: Anis.Feki@h-fr.ch.
² Service of Genetic Medicine, Geneva University Hospitals, 4 Rue Gabrielle-Perret-Gentil, 1211 Geneva 4, Switzerland.
³ Service de gynécologie obstétrique, HFR Fribourg - Hôpital cantonal, Chemin des Pensionnats 2-6, Case postale, 1708 Fribourg, Switzerland; Stem Cell Research Laboratory, Department of Obstetrics and Gynecology, Geneva University Hospitals, Geneva, Switzerland. Electronic address: youssef.hibaoui@unifr.ch.

PMID: 32739879
DOI: 10.1016/j.scr.2020.101920

Abstract

Turner syndrome, caused by partial or complete loss of one copy of X-chromosome (45,X), is the most common sex chromosome abnormality in women with an incidence of 1 in 2500 female births. Here, we report the generation and characterization of induced pluripotent stem cells (iPSCs) carrying X-monosomy anomaly, with isogenic control iPSCs. Among the iPSC lines generated from 46XX-fibroblasts, one spontaneously lost a copy of X-chromosome following the reprogramming process, establishing the 45X-iPSC line.