Genome-wide identification and characterization of HSP70 gene family in four species of cotton

Abdul Rehman; Rana Muhammad Atif; Abdul Qayyum; Xiongming Du; Lori Hinze; Muhammad Tehseen Azhar

doi:10.1016/j.ygeno.2020.07.039

Genome-wide identification and characterization of HSP70 gene family in four species of cotton

Genomics. 2020 Nov;112(6):4442-4453. doi: 10.1016/j.ygeno.2020.07.039. Epub 2020 Jul 31.

Authors

Abdul Rehman¹, Rana Muhammad Atif², Abdul Qayyum³, Xiongming Du⁴, Lori Hinze⁵, Muhammad Tehseen Azhar⁶

Affiliations

¹ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research Chinese Academy of Agricultural Science, Anyang, Henan 455000, China; Department of Plant Breeding and Genetics, Bahauddin Zakariya University, Multan 66000, Pakistan.
² Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38040, Pakistan; Center of Advanced Studies in Agriculture & Food Security, University of Agriculture, Faisalabad 38040, Pakistan.
³ Department of Plant Breeding and Genetics, Bahauddin Zakariya University, Multan 66000, Pakistan.
⁴ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research Chinese Academy of Agricultural Science, Anyang, Henan 455000, China. Electronic address: duxiongming@caas.cn.
⁵ USDA, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX 77845, USA.
⁶ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China; Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38040, Pakistan. Electronic address: tehseenazhar@gmail.com.

PMID: 32739432
DOI: 10.1016/j.ygeno.2020.07.039

Abstract

Heat shock proteins (HSPs) are important elements of the cellular group of molecular chaperones. Specifically, HSP70 proteins protect cells from being damaged when plants are exposed to environmental stresses. These proteins are catalysts that manage the correct folding of other proteins, and they play a key role in the development of tolerance against biotic and abiotic stresses. In the present study, 113 HSP70 genes were retrieved from the available genome assemblies of four cotton species, including Gossypium hirsutum, G. barbadense, G. arboreum, and G. raimondii. The HSP70 genes were clustered into 11 subfamilies based on phylogeny. One hundred and nine (109) gene duplications were found across these four species. Localization of genes revealed that several HSP70 genes reside in the cytoplasm. Synonymous and non-synonymous substitution rates revealed that functional segregation of HSP70 genes in cotton is due to purifying selection. Furthermore, HSP70 genes in cotton are expressed constitutively during developmental stages. These findings are valuable to understand the complex mechanism of HSP70 gene regulation that occurs in signaling pathways in response to plant stress.

Keywords: Cellular localization; Cotton; HSP70; Heat shock protein; Substitution rate.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Motifs
Chromosome Mapping
Conserved Sequence
Gene Duplication
Genes, Plant
Genome, Plant
Gossypium / genetics*
Gossypium / metabolism
HSP70 Heat-Shock Proteins / chemistry
HSP70 Heat-Shock Proteins / classification
HSP70 Heat-Shock Proteins / genetics*
HSP70 Heat-Shock Proteins / metabolism
Multigene Family*
Phylogeny
Plant Proteins / chemistry
Plant Proteins / classification
Plant Proteins / genetics*
Plant Proteins / metabolism

Substances

HSP70 Heat-Shock Proteins
Plant Proteins