Enhanced Cd2+ and Zn2+ removal from heavy metal wastewater in constructed wetlands with resistant microorganisms

Abstract

The bioaugmentation role of microbes is often impeded by heavy metal (HM) ions in constructed wetlands (CWs). To explore the interaction between microbes and HM ions, two identical CWs: an MCW (with resistant microorganisms) and a CCW (as control) were used in this study. Experiments analyzed static adsorption performance in a synthetic HM solution. The removal performance of Cd²⁺ and Zn²⁺ was further investigated in both CWs. The removal efficiencies (REs) of 81.92-99.56% and 74.05-98.79% were achieved for Cd²⁺ and Zn²⁺ in the adsorption study, respectively. Significantly higher REs of Cd²⁺ (99.60%), and Zn²⁺ (94.41%) were achieved in the MCW. The microbial community analysis revealed that the dominant genera were Serratia and Pseudomonas in the MCW. The subcellular analysis further demonstrated that the HMs bioaccumulated mainly in the cytomembrane and cell wall. These results indicate that CW with resistant microorganisms inoculated was an effective strategy for treating HMs wastewater.

Keywords: Bioaugmentation; Constructed wetlands; Heavy metals; Microorganisms; Wastewater treatment.