Typing of Campylobacter jejuni isolated from poultry on the basis of fla A-RFLP by various restriction enzymes

Rahul Yadav; Jyotika Yadav; Sunil Maherchandani; Sudhir Kumar Kashyap

doi:10.1016/j.vas.2018.06.003

Typing of Campylobacter jejuni isolated from poultry on the basis of fla A-RFLP by various restriction enzymes

Vet Anim Sci. 2018 Jul 4:6:1-5. doi: 10.1016/j.vas.2018.06.003. eCollection 2018 Dec.

Authors

Rahul Yadav¹, Jyotika Yadav², Sunil Maherchandani¹, Sudhir Kumar Kashyap¹

Affiliations

¹ Department of Veterinary Microbiology and Biotechnology, CVAS, RAJUVAS, Bikaner, Rajasthan, India.
² Department of Veterinary Medicine, COVS, LUVAS, Hisar, Haryana, India.

Abstract

RFLP analysis of the flagellin (flaA) gene was compared using three different restriction endonucleases i.e DdeI, HinfI and DpnII to determine the genetic diversity among 43 Campylobacter jejuni isolates of poultry origin from the same geographical area. flaA gene was amplified in all the isolates and RFLP analysis showed variations. Dde-based RFLP was found most efficient in discriminating C. jejuni isolates by generating 15 different Dde-RFLP patterns with discriminatory index (D.I) of 0.9258 whereas DpnII produced seven Dpn-RFLP patterns (D.I .= 0.8427). While HinfI enzyme produced only six Hinf-RFLP patterns (D.I .= 0.6977). The discrimination of Dpn-RFLP was comparable to discrimination given by Dde-RFLP analysis, which is generally used to study flaA gene RFLP.

Keywords: C. jejuni; Poultry; ddeI; dpnII; flaA RFLP.