Protection of Piglets with Maternally Derived Antibodies from Sows Inoculated with an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BErns)

SeEun Choe; Jihye Shin; Ki-Sun Kim; Sok Song; Ra Mi Cha; Byung-Il Jung; Bang-Hun Hyun; Bong-Kyun Park; Dong-Jun An

doi:10.3390/pathogens9080608

Protection of Piglets with Maternally Derived Antibodies from Sows Inoculated with an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BE^rns)

Pathogens. 2020 Jul 27;9(8):608. doi: 10.3390/pathogens9080608.

Authors

SeEun Choe¹, Jihye Shin¹, Ki-Sun Kim¹, Sok Song¹, Ra Mi Cha¹, Byung-Il Jung², Bang-Hun Hyun¹, Bong-Kyun Park^{1

3}, Dong-Jun An¹

Affiliations

¹ Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea.
² Korea Pork Producers Association, Seocho-gu, Seoul 06643, Korea.
³ College of Veterinary Medicine, Seoul University, Gwanak-ro, Gwanak-gu, Seoul 08826, Korea.

Abstract

Here, we investigated the protective efficacy provided by passive immunity induced by a classical swine fever (Flc-LOM-BE^rns) vaccine with the newly developed DIVA (Differentiating Infected from Vaccinated Animals) function. Ten pigs (aged 40-60 days) with maternally derived antibodies (MDAs) obtained from sows inoculated with the Flc-LOM-BE^rns vaccine were challenged with virulent classical swine fever virus (CSFV). Pigs with an MDA titer of 6 log₂ induced by the Flc-LOM-BE^rns vaccine were fully protected against virulent CSFV challenge but not the pigs with an MDA titer under 5 log₂. In addition, Flc-LOM-BE^rns vaccine-derived MDAs successfully differentiated vaccinated pigs by bovine viral diarrhea virus (BVDV) E^rns/CSFV E^rns antibody detection, functioning as a DIVA.

Keywords: CSFV; DIVA; Flc-LOM-BErns vaccine; MDA.

Grants and funding

B-1543083-2019-20-01/Animal and Plant Quarantine Agency