Fabrication and Characterization of Ceftizoxime-Loaded Pectin Nanocarriers

Pawan Kumar; Vinod Kumar; Ravinder Kumar; Catalin Iulian Pruncu

doi:10.3390/nano10081452

Fabrication and Characterization of Ceftizoxime-Loaded Pectin Nanocarriers

Nanomaterials (Basel). 2020 Jul 24;10(8):1452. doi: 10.3390/nano10081452.

Authors

Pawan Kumar¹, Vinod Kumar², Ravinder Kumar³, Catalin Iulian Pruncu^{4

5}

Affiliations

¹ Department of Materials Science and Nanotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India.
² Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar 125001, India.
³ Department of Mechanical Engineering, Lovely Professional University, Phagwara 144411, India.
⁴ Mechanical Engineering Department, University of Birmingham, Birmingham B15 2TT, UK.
⁵ Mechanical Engineering, Imperial College London, Exhibition Rd., London SW7 2AZ, UK.

Abstract

Ceftizoxime (C₁₃H₁₂N₅NaO₅S₂)is a parenteral, third-generationcephalosporin antibiotic used to treat bacterial infections including ear, nose, and throat infections. In this work, pectin has been used as a nanocarrier for ceftizoxime due to its high biocompatibility and non-toxicity with tunable surface properties. Ceftizoxime-loaded pectin nanocarriers (CPN) were successfully synthesized by the solvent displacement method. Optimization of nanoformulation was done by response surface methodology using Design-Expert software. The optimized formulation examined various in-vitro characterizations such as particle size, morphology, and FTIR studies. TEM results revealed irregular shape nanoparticles within the range of 29-110 nm. The in-vitro drug release using the dialysis method was performed after 24h where nanoformulation showed sustained drug release. Drug-loaded nanoparticles revealed good antimicrobial activity against Bacillus cereus, Bacilluspolymyxa, Enterobacteraerogenes, andPseudomonasaeruginosa.

Keywords: Ceftizoxime; antibacterial activity; nanocarriers; pectin; solvent displacement.