Injectable bone substitute based on chitosan with polyethylene glycol polymeric solution and biphasic calcium phosphate microspheres

Daniel Bezerra Lima; Mônica Adriana Araújo de Souza; Gabriel Goetten de Lima; Erick Platiní Ferreira Souto; Hugo Miguel Lisboa Oliveira; Marcus Vinícius Lia Fook; Marcelo Jorge Cavalcanti de Sá

doi:10.1016/j.carbpol.2020.116575

Injectable bone substitute based on chitosan with polyethylene glycol polymeric solution and biphasic calcium phosphate microspheres

Carbohydr Polym. 2020 Oct 1:245:116575. doi: 10.1016/j.carbpol.2020.116575. Epub 2020 Jun 10.

Authors

Daniel Bezerra Lima¹, Mônica Adriana Araújo de Souza², Gabriel Goetten de Lima³, Erick Platiní Ferreira Souto², Hugo Miguel Lisboa Oliveira⁴, Marcus Vinícius Lia Fook¹, Marcelo Jorge Cavalcanti de Sá⁵

Affiliations

¹ Unidade Académica de Engenharia dos Materiais - CERTBIO, Universidade Federal de Campina Grande, Campina Grande, Paraíba, Brazil.
² Programa de Pós-Graduação em Medicina Veterinária - PPGMV, Universidade Federal de Campina Grande, Campina Grande, Paraíba, Brazil.
³ Programa de Pós-Graduação em Engenharia e Ciência dos Materiais - PIPE, Universidade Federal do Paraná, Curitiba, Paraná, Brazil; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland.
⁴ Unidade Académica de Engenharia dos Alimentos, Universidade Federal de Campina Grande, Campina Grande, Paraíba, Brazil.
⁵ Programa de Pós-Graduação em Medicina Veterinária - PPGMV, Universidade Federal de Campina Grande, Campina Grande, Paraíba, Brazil; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland. Electronic address: mjcdesa@gmail.com.

PMID: 32718652
DOI: 10.1016/j.carbpol.2020.116575

Abstract

We described a method to produce an injectable bone substitute consisting of a solid and liquid phase, this solid was formed using the coacervation method consisting of a mixture of Hydroxyapatite (HAp) and beta-Tricalcium Phosphate (β-TCP) which the sodium alginate - precursor - was removed during sinterization. The biphasic calcium phosphate microspheres had varying size distributions depending on the flow rate and these microspheres were mixed with a polymeric solution, chitosan and polyethylene glycol, and depending on the ratio of these phases, the injectability results varied. Nonetheless, the force required for complete removal will not disrupt the accuracy of injection into the bone defect while the biomaterial exhibited no cytotoxicity with promising results from in vivo using tibia bone defect in rabbits at 30 and 60 days whereas bone repair was more intense and accentuated with the usage of the biomaterial, and was gradually absorbed during the evaluated periods.

Keywords: Biphasic calcium phosphate; Injectable bone substitute; Microspheres; Tibial bone defect.