Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro

Xiao-Hong Du; Qing-Jun Chen; Jian-Bo Song; Yan Xie; Yan Zhi; Ru-Ru Sun; Guo-Hui Liu; Xin Kang

doi:10.1155/2020/5834502

Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro

Evid Based Complement Alternat Med. 2020 Jul 4:2020:5834502. doi: 10.1155/2020/5834502. eCollection 2020.

Authors

Xiao-Hong Du¹, Qing-Jun Chen², Jian-Bo Song³, Yan Xie⁴, Yan Zhi⁵, Ru-Ru Sun², Guo-Hui Liu⁶, Xin Kang²

Affiliations

¹ Department of Hepatobiliary Pancreatic Surgery, First Hospital Bethune of Jilin University, Changchun 130021, China.
² Department of Emergency Medicine, Beijing Hepingli Hospital, Beijing 100013, China.
³ Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian University, Dalian 116001, China.
⁴ Organ Transplant Center, Tianjin First Central Hospital, Tianjin 300000, China.
⁵ Department of Burns, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China.
⁶ Department of Emergency Medicine, The First Hospital of Jilin University, Changchun 130012, China.

Abstract

Rhubarb-Aconite Decoction (RAD), a famous Chinese medicine prescription, has been widely used for treating intestinal injury. However, the effect of RAD on intestinal epithelial cells is unclear. The aim of this study was to investigate the effects of RAD drug-containing serum on the oxidative stress injury and inflammatory response induced by endotoxin (ET) in Caco-2 cells in vitro. Lipid peroxide malondialdehyde (MDA), lactate dehydrogenase (LDH), caspase-11, tumor necrosis factor-α(TNF-α), interleukin-3(IL-3), and cytokeratin (CK)18, adenosine triphosphate (ATP) activity, and intracellular free calcium ion levels were measured. The results showed that ET triggered the activation of caspase-11 and the massive release of TNF-α, increased the inhibitory rate of cell growth, MDA, and LDH expressions in Caco-2 cells. Moreover, RAD drug-containing serum could inhibit caspase-11 activation, decrease the release of TNF-α and IL-3, reduce intracellular free calcium ion, and enhance CK 18 expression and ATP activity. These novel findings demonstrated that ET-induced oxidative stress injury and inflammatory response of Caco-2 cells were improved by RAD drug-containing serum, indicating that RAD may be a good choice for the treatment of intestinal injury.