Interaction of HaNPVs with two novel insecticides against Helicoverpa armigera Hubner (Noctuidae: Lepidoptera)

Saudi J Biol Sci. 2020 Aug;27(8):2124-2128. doi: 10.1016/j.sjbs.2020.06.023. Epub 2020 Jun 20.

Abstract

Nucleopolyhedrosis viruses can be utilized for effective management of agriculture pests. Their efficacy can be increased if they are mixed with certain insecticides. In the current study, HaNPV was mixed with two insecticides: spinetoram and emamectin benzoate in various combinations and applied to larvae of H. armigera in laboratory conditions. There were a total of 15 combinations of HaNPV with each of the two insecticides in addition to five doses of HaNPV and three doses of insecticides alone. The synergistic and antagonistic effects of combinations were explored. The results revealed that there was synergistic effect of HaNPV @ 0.5 × 109 PIB/ml × Spinetoram @ 40, 20, 10 ml/100 L of water. In case of emamectin benzoate, synergistic effects were recorded at 1 × 109 PIB/ml HaNPV × emamectin benzoate @ 100 ml/100 L of water. However, 0.5 × 109 PIB/ml HaNPV has synergistic effects with all three doses of emamectin benzoate. The results suggested that HaNPV can be used in combination with spinetoram and emamectin benzoate for the management of resistant population of H. armigera.

Keywords: Combination; Emamectin benzoate; HaNPV; Spinetoram; Synergistic effect.