In vitro bioaccessibility of metals from tape tea - A low-cost emerging drug

Aline Martins de Andrade; Rodrigo Moretto Galazzi; Manuel Miró; Marco Aurélio Zezzi Arruda

doi:10.1016/j.jtemb.2020.126613

In vitro bioaccessibility of metals from tape tea - A low-cost emerging drug

J Trace Elem Med Biol. 2020 Dec:62:126613. doi: 10.1016/j.jtemb.2020.126613. Epub 2020 Jul 17.

Authors

Aline Martins de Andrade¹, Rodrigo Moretto Galazzi¹, Manuel Miró², Marco Aurélio Zezzi Arruda³

Affiliations

¹ Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
² FI-TRACE Group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa km 7.5, E-07122 Palma de Mallorca, Spain.
³ Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil. Electronic address: zezzi@unicamp.br.

PMID: 32712520
DOI: 10.1016/j.jtemb.2020.126613

Abstract

Background: An in vitro physiologically relevant test based on the standard Unified Bioaccessibility Method (UBM) combined with inductively coupled plasma mass spectrometry was performed in this study to ascertain the elemental bioaccessibility pools of tape tea as emerging low-cost abuse drug under fasted conditions.

Methods: Elemental quantification in tape tea and body fluid extracts was performed by an inductively coupled plasma quadrupole mass spectrometer - ICP-MS, and for sample preparation of the bioaccessibility extracts prior to ICP-MS analysis, a microwave-assisted acid decomposition was applied by using a microwave oven. The Unified Bioaccessibility Method (UBM) was considered for investigation of elemental bioaccessibility in tape tea, required a full set of organic compounds, salts, and enzymes.

Results: Considering total element evaluation through ICP-MS, Co, Ni, Mn, and Zn are found at the highest concentrations in the sample, namely 415 ± 36, 202 ± 55, 1389 ± 225 and 2397 ± 197 μg L^-1, respectively. Regarding the oral bioaccessibility test, after both gastric and gastrointestinal extractions Co, Ni, and Mn are fully bioaccessible while for Zn the bioaccessibility is ca. 66 %.

Conclusion: According to the first results in the literature proposed for these samples, the bioaccessibility results indicate an increment in day-to-day total element concentration and depending on the concentration of each element that an individual consumes in its usual diet, the total concentration can exceed the TDI. There are several possible toxic effects caused by the excess of Co, Ni and Mn, which might be expected by their high total concentrations.

Keywords: ICP-MS; Low-cost drugs; Mass spectrometry; Metal bioaccessibility; Tape tea.

MeSH terms

Animals
Gastrointestinal Tract / metabolism
Humans
Manganese / analysis
Mass Spectrometry / methods*
Metals / analysis
Microwaves
Nickel / analysis
Trace Elements / analysis
Zinc / analysis

Substances

Metals
Trace Elements
Manganese
Nickel
Zinc