T Cell Receptor Repertoire Sequencing

Huixin Lin; Yonggang Peng; Xiangbin Chen; Yuebin Liang; Geng Tian; Jialiang Yang

doi:10.1007/978-1-0716-0904-0_1

T Cell Receptor Repertoire Sequencing

Methods Mol Biol. 2020:2204:3-12. doi: 10.1007/978-1-0716-0904-0_1.

Authors

Huixin Lin¹, Yonggang Peng¹, Xiangbin Chen¹, Yuebin Liang¹, Geng Tian¹, Jialiang Yang²

Affiliations

¹ Geneis (Beijing) Co., Ltd., Beijing, People's Republic of China.
² Geneis (Beijing) Co., Ltd., Beijing, People's Republic of China. yangjl@geneis.cn.

PMID: 32710310
DOI: 10.1007/978-1-0716-0904-0_1

Abstract

The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.

Keywords: Data analysis; High-throughput sequencing; Library construction; T cell receptor repertoire.

Publication types

Review

MeSH terms

Cloning, Molecular / methods
Complementarity Determining Regions / genetics
Gene Library
High-Throughput Nucleotide Sequencing / methods*
Humans
Receptors, Antigen, T-Cell / genetics*
Receptors, Antigen, T-Cell, alpha-beta / genetics
T-Lymphocytes / immunology
T-Lymphocytes / physiology

Substances

Complementarity Determining Regions
Receptors, Antigen, T-Cell
Receptors, Antigen, T-Cell, alpha-beta