Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

Md Sharifull Islam; Yang Hu; Md Furkanur Rahaman Mizan; Ting Yan; Ishatur Nime; Yang Zhou; Jinquan Li

doi:10.3390/microorganisms8071089

Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

Microorganisms. 2020 Jul 21;8(7):1089. doi: 10.3390/microorganisms8071089.

Authors

Md Sharifull Islam^{1

2

3}, Yang Hu⁴, Md Furkanur Rahaman Mizan⁵, Ting Yan³, Ishatur Nime³, Yang Zhou^{1

2}, Jinquan Li^{2

3

6}

Affiliations

¹ College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
² State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
³ Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
⁴ Division of Microbiology, Brewing and Biotechnology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
⁵ Department of Food Science and Technology, Chung-Ang University, Anseong, Gyunggido 456-756, Korea.
⁶ Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10065-6399, USA.

Abstract

Foodborne diseases represent a major risk to public health worldwide. In this study, LPST153, a novel Salmonella lytic phage with halo (indicative of potential depolymerase activity) was isolated by employing Salmonella enterica serovar Typhimurium ATCC 13311 as the host and had excellent lytic potential against Salmonella. LPST153 is effectively able to lyse most prevalent tested serotypes of Salmonella, including S. Typhimurium, S. Enteritidis, S. Pullorum and S. Gallinarum. Morphological analysis revealed that phage LPST153 belongs to Podoviridae family and Caudovirales order and could completely prevent host bacterial growth within 9 h at multiplicity of infection (MOI) of 0.1, 1, 10 and 100. LPST153 had a latent period of 10 min and a burst size of 113 ± 8 PFU/cell. Characterization of the phage LPST153 revealed that it would be active and stable in some harsh environments or in different conditions of food processing and storage. After genome sequencing and phylogenetic analysis, it is confirmed that LPST153 is a new member of the Teseptimavirus genus of Autographivirinae subfamily. Further application experiments showed that this phage has potential in controlling Salmonella in milk and sausage. LPST153 was also able to inhibit the formation of biofilms and it had the ability to reduce and kill bacteria from inside, including existing biofilms. Therefore, the phage LPST153 could be used as a potential antibacterial agent for Salmonella control in the food industry.

Keywords: LPST153; Salmonella; biofilm; food; halo; phage.

Abstract

Grants and funding