In this study, Bacillus coagulans CC17A with highly tolerant to hydrolysate was obtained through adaptive evolution. After 63 generations, the strain CC17A was stably in 45% (v/v) hydrolysate media and could digest multiple inhibitors in the hydrolysate. Based on its promising features, a one-pot process was developed to produce lactic acid (LA) from wheat straw. After dilute acid pretreatment of wheat straw, simultaneous saccharification and co-fermentation was conducted using CC17A without any solid-liquid separation and pre-detoxification. Total 35.50 g LA was produced from 80 g raw substrate and the production yield was as high as 70.9% of theoretical. To elucidate the tolerance mechanism, transcriptomic profiling of CC17A was studied. The highly up-regulated oxidoreductases and phenolic acid decarboxylase are considered to be involved with the inhibitors-tolerance of B. coagulans CC17A.
Keywords: Adaptive evolution; Bacillus coagulans; Hydrolysate-tolerance; One-pot process; Transcriptome.
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