Effect of maternal diet on select fecal bacteria of foals

Morgan B Pyles; Ashley L Fowler; Veronica T Bill; Brittany E Harlow; Andrea D Crum; Susan H Hayes; Michael D Flythe; Laurie M Lawrence

doi:10.1093/tas/txy141

Effect of maternal diet on select fecal bacteria of foals

Transl Anim Sci. 2018 Dec 24;3(1):204-211. doi: 10.1093/tas/txy141. eCollection 2019 Jan.

Authors

Morgan B Pyles¹, Ashley L Fowler¹, Veronica T Bill¹, Brittany E Harlow^{1

2}, Andrea D Crum¹, Susan H Hayes¹, Michael D Flythe^{1

2}, Laurie M Lawrence¹

Affiliations

¹ Department of Animal and Food Sciences, University of Kentucky, Lexington, KY.
² Forage-Animal Production Research Unit, Agricultural Research Service, United States Department of Agriculture, Lexington, KY.

Abstract

Adult horses depend on the microbial community in the hindgut to digest fiber and produce short-chain fatty acids that are use for energy. Colonization of the foal gastrointestinal tract is essential to develop this symbiosis. However, factors affecting colonization are not well understood. The objectives of this study were to evaluate the age-related changes and effects of maternal diet on select fecal bacterial groups in foals from 1 to 28 d of age. Thoroughbred foals (n = 18) were from dams fed forage and one of two concentrates: an oat-based (OB) or corn and wheat middlings-based (CWB) pelleted concentrate. The mares had access to assigned concentrates, along with a mixed hay and cool-season grass pasture, 28 d before and 28 d after parturition. Fecal samples were collected from foals at 1 d (14 to 36 h), 4, 14, and 28 d after birth. Fecal samples were serially diluted with phosphate-buffered saline before inoculation of enriched, selective media to enumerate Lactobacillus spp., amylolytic bacteria, and cellulolytic bacteria. Enumeration data were log-transformed then analyzed with mixed model analysis of variance with repeated measures (SAS 9.3) to test the main effects of maternal diet (OB or CWB), time of sample, and interaction between maternal diet and time. Cellulolytic bacteria first appeared in foal feces between 4 and 14 d of age and increased with age (P < 0.05). Amylolytic bacteria and lactobacilli were abundant at 1 d and then increased with age (P < 0.05). There was an interaction between maternal diet and time for Lactobacillus spp. with OB foals having more lactobacilli than CWB foals at 1 and 4 d (P < 0.05); however, there were no differences observed at 14 d (P > 0.05). Maternal diet did not influence amylolytic or cellulolytic bacteria (P > 0.05). These results indicate that colonization of the hindgut is a sequential process beginning early in the foal's life and that maternal diet may influence some bacteria in the gastrointestinal tract of foals.

Keywords: horse; microbial colonization; starch source.