DNA based biosensing of Acinetobacter baumannii using nanoparticles aggregation method

Farnaz Bahavarnia; Paria Pashazadeh-Panahi; Mohammad Hasanzadeh; Nasrin Razmi

doi:10.1016/j.heliyon.2020.e04474

DNA based biosensing of Acinetobacter baumannii using nanoparticles aggregation method

Heliyon. 2020 Jul 16;6(7):e04474. doi: 10.1016/j.heliyon.2020.e04474. eCollection 2020 Jul.

Authors

Farnaz Bahavarnia^{1

2

3

4}, Paria Pashazadeh-Panahi^{5

6

7}, Mohammad Hasanzadeh⁸, Nasrin Razmi⁹

Affiliations

¹ Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
² Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁵ Hematology-Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran.
⁶ Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁷ Endocrinology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁸ Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁹ Department of Science and Technology, Physics, Electronics and Mathematics, Linköping University, Sweden.

Abstract

Acinetobacter baumannii is the main cause of nosocomial infections in blood, urinary tract, wounds and in lungs leading to pneumonia. Apart from its strong predilection to be the cause of serious illnesses in intensive care units. Herein, we present a specific and sensitive approach for the monitoring of Acinetobacter baumannii genome based on citrate capped silver nanoparticles (Cit-AgNPs) using spectroscopic methods. In this study, (5' SH-TTG TGA ACT ATT TAC GTC AGC ATG C3') sequence was used as a probe DNA (pDNA) of Acinetobacter baumannii. Then, complementary DNA (cDNA) was used for hybridization. After the hybridization of pDNA with cDNA, target DNA (5' GCA TGC TGA CGT AAA TAGTTC ACA A 3') was recognized and detected using turn-on fluorescence bioassay. After the hybridization of pDNA with cDNA, the target DNA was successfully measured in optimum time of 2 min by spectrophotometric techniques. Moreover, the selectivity of designed bioassay was evaluated in the presence of two mismatch sequences and excellent differentiation was obtained. 1 Zepto-molar (zM) of low limit of quantification (LLOQ) was achieved by this genosensor. The present study paved the way for quick (2 min) and accurate detection of Acinetobacter baumannii, which can be a good alternative to the traditional methods. Current study proposed a novel and significant diagnostic test towards Acinetobacter baumannii detection based on silver nanoparticles aggregation which has the capability of being a good alternative to the traditional methods. Moreover, the proposed genosensor successfully could be applied for the detection of other pathogens.

Keywords: Acinetobacter baumannii; Analytical chemistry; Biosensor; Chemistry; DNA hybridization; Genosensor; Microbiology; Nanoparticles; Pathogenic bacteria; Sensor.