Japanese mushroom consumption alters the lipid metabolomic profile of high-fat diet-fed mice

Takamitsu Shimizu; Koichiro Mori; Hitoshi Kobayashi; Tsuyoshi Tsuduki

doi:10.1016/j.heliyon.2020.e04438

Japanese mushroom consumption alters the lipid metabolomic profile of high-fat diet-fed mice

Heliyon. 2020 Jul 15;6(7):e04438. doi: 10.1016/j.heliyon.2020.e04438. eCollection 2020 Jul.

Authors

Takamitsu Shimizu¹, Koichiro Mori¹, Hitoshi Kobayashi¹, Tsuyoshi Tsuduki²

Affiliations

¹ Mushroom Research Laboratory, Hokuto Corporation, Nagano 381-0008, Japan.
² Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 980-0845, Japan.

Abstract

Mushrooms are familiar ingredients in Japanese cuisine and large numbers are consumed in Japan. Recently, we reported that the consumption of Japanese mushrooms suppressed the accumulation of visceral fat. The purpose of this study was to examine the alteration of lipid metabolism by Japanese mushrooms consumption in high-fat diet (HFD) mice. Multivariate analysis of serum, liver, adipose tissue, cecal contents, large intestinal and fecal lipids showed differing compositions in the mice that had consumed HFD or HFD supplemented with 3% freeze-dried mushroom mixture (HFMD). There were higher concentrations of diacylglycerol in the adipose tissue, non-esterified fatty acids in the serum, and triacylglycerol in the feces of the HFMD group. These results suggest that mushroom consumption promotes the degradation of lipids in visceral fat and limits the absorption of food lipids. Moreover, the HFMD group demonstrated higher concentrations of phospholipids, some of which contained odd-chain fatty acids. Thus, we speculated that the alteration of lipid metabolism in mice such that mushroom consumption prevent obesity progression, as demonstrated by metabolomic analysis.

Keywords: Food science; Japanese mushroom; Lipid profile; Metabolome; Obesity.