Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Amy Strydom; Celeste Donato; Ina Peenze; A Christiaan Potgieter; Mapaseka Seheri; Hester G O'Neill

doi:10.1016/j.meegid.2020.104463

Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Infect Genet Evol. 2020 Nov:85:104463. doi: 10.1016/j.meegid.2020.104463. Epub 2020 Jul 18.

Authors

Amy Strydom¹, Celeste Donato², Ina Peenze³, A Christiaan Potgieter⁴, Mapaseka Seheri³, Hester G O'Neill⁵

Affiliations

¹ Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
² Enteric Diseases Group, Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, The University of Melbourne, Parkville, Australia; Biomedicine Discovery Institute and Department of Microbiology, Monash University, Melbourne, Australia.
³ Diarrhoeal Pathogens Research Unit, Department of Virology, Sefako Makgatho Health Sciences University, Pretoria, South Africa.
⁴ Department of Biochemistry, Focus Area Human Metabolomics, North-West University, Potchefstroom, South Africa; Deltamune (Pty) Ltd, Moraine house - The Braes, 193 Bryanston Drive, Bryanston, Gauteng 2191, South Africa.
⁵ Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa. Electronic address: OneillHG@ufs.ac.za.

PMID: 32693063
DOI: 10.1016/j.meegid.2020.104463

Abstract

We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.

Keywords: African buffalo; Artiodactyl backbone; G29; P[14]; Rotavirus type A.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Buffaloes / virology*
Cattle
Feces / virology
Genome, Viral
Genotype
Phylogeny
RNA, Viral
Rotavirus / classification
Rotavirus / genetics*
Rotavirus Infections / epidemiology
Rotavirus Infections / veterinary*
Rotavirus Infections / virology*
South Africa / epidemiology

Substances

RNA, Viral