Photoprotective effect of Astragalus membranaceus polysaccharide on UVA-induced damage in HaCaT cells

Qiong Li; Depeng Wang; Donghui Bai; Chao Cai; Jia Li; Chengxiu Yan; Shuai Zhang; Zhijun Wu; Jiejie Hao; Guangli Yu

doi:10.1371/journal.pone.0235515

Photoprotective effect of Astragalus membranaceus polysaccharide on UVA-induced damage in HaCaT cells

PLoS One. 2020 Jul 21;15(7):e0235515. doi: 10.1371/journal.pone.0235515. eCollection 2020.

Authors

Qiong Li^{1

2}, Depeng Wang^{1

2}, Donghui Bai^{1

2}, Chao Cai^{1

2

3}, Jia Li^{1

2}, Chengxiu Yan^{1

2}, Shuai Zhang^{1

2}, Zhijun Wu⁴, Jiejie Hao^{1

2

3}, Guangli Yu^{1

2

3}

Affiliations

¹ Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
² Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
³ Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
⁴ Infinitus (China) Company Ltd., Guangzhou, China.

Abstract

Background: The skin provides a predominant barrier against chemical, physical and microbial incursion. The intemperate exposure to ultraviolet A (UVA) radiation can cause excessive cellular oxidative stress, leading to skin damage, proteins damage and mitochondrial dysfunction. There is sufficient evidences supporting the proposal that mitochondria is highly implicated in skin photo-damage.

Methods: In the present study, a polysaccharide isolated from Astragalus membranaceus was further purified to be an α-glucan, which was further investigated its beneficial influence on UVA-induced photo-damage in HaCaT cells.

Results: Our results showed that the purified Astragalus membranaceus polysaccharide (AP) can protect HaCaT cells from UVA-induced photo-damage through reducing UVA-induced intracellular ROS production and mitochondrial membrane potential, thereby altering ATP content. It was found that the UVA induced damage in HaCaT cells could be effectively restored by co-treatment with AP.

Conclusions: AP exhibited promising potential for advanced application as multifunctional skin care products and drugs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / metabolism
Apoptosis / drug effects
Astragalus propinquus / chemistry*
Cell Line
Cell Survival / drug effects
Humans
Keratinocytes / cytology
Keratinocytes / drug effects*
Keratinocytes / metabolism
Keratinocytes / radiation effects*
Membrane Potential, Mitochondrial / drug effects
Oxidative Stress / drug effects
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Radiation-Protective Agents / chemistry
Radiation-Protective Agents / pharmacology*
Reactive Oxygen Species / metabolism
Ultraviolet Rays / adverse effects*

Substances

Polysaccharides
Radiation-Protective Agents
Reactive Oxygen Species
Adenosine Triphosphate

Grants and funding

This work was supported by National Natural Science Foundation of China (81973231, 81402982), National Science and Technology Major Project for Significant New Drugs Development (2018ZX09735004), Taishan Scholar Project Special Fund (TS201511011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.