Triterpenoids isolated from Jatropha macrantha (Müll. Arg.) inhibit the NF-κB and HIF-1α pathways in tumour cells

Abstract

Activity-guided fractionations of Jatropha macrantha Müll. Arg. led to the isolation of pomolic acid (1) and euscaphic acid (2). The potential for inhibition against NF-κB and HIF-1α production of these two compounds was tested in different tumour cell lines. Compounds 1 and 2 showed an inhibitory activity of HIF-1α in the SK-MEL-28 (IC₅₀=3.01 ± 0.02 μM and 3.78 ± 0.02 μM), A549 (IC₅₀=9.97 ± 0.01 μM and 10.25 ± 0.01 μM) and U-373 MG (IC₅₀=6.34 ± 0.02 μM and 8.85 ± 0.02 μM) cell lines. In addition, compounds 1 and 2 showed an inhibitory activity on NF-κB in SK-MEL-28 (IC₅₀=1.05 ± 0.02 μM and 2.71 ± 0.01 μM), A549 (IC₅₀=3.63 ± 0.01 μM and 3.73 ± 0.02 μM) and U-373 MG (IC₅₀=2.55 ± 0.02 μM and 3.39 ± 0.01 μM) cell lines. This is the first report that isolates these compounds from J. macrantha and tests their antitumor potential.

Keywords: Euphorbiaceae; HIF-1α; Jatropha macrantha; NF-κB; Sterols; Triterpenoids.