Phosphate (P) fertilization is a commonly used agronomic practice. However, research on bioremediation is very limited. This study's principal objective was to evaluate the role of P in the growth and heavy metals (HMs) accumulation of Polygonum pubescens Blume cultured in Mn-contaminated soil. To this end, the effects of sodium dihydrogen phosphate (SDP) and single superphosphate (SSP) on the growth, Mn bioremediation efficiency, organ HMs, and physiological parameters related to antioxidant stress of P. pubescens were examined. The results showed that both SDP and SSP increased soil pH and available P but decreased available HMs. Phosphate significantly (P < 0.05) promoted P. pubescens height and biomass. Average height increased by 36.1% and 32.6% with SDP and SSP, respectively, with corresponding biomass increases of 71.8% and 135%. Phosphate significantly (P < 0.05) reduced Mn concentrations, especially in leaves, where the values decreased by >50.0% for DSP and SSP. Total Mn significantly (P < 0.05) decreased with DSP amendment but significantly (P < 0.05) increased by 38.5% with SSP (200 mg kg-1) through an increase in biomass. Phosphate significantly (P < 0.05) decreased all organ HM concentrations and translocation, indicating that less HM stress occurred with P amendment. The changes in reactive oxygen species, antioxidants and non-antioxidant materials further supported these results. Pearson correlation analysis revealed negative relationships between soil available P and HMs, indicating a novel role of P in HM passivation. The uncommonly high Ca concentrations in leaves suggested that Ca plays a vital role in promoting growth and alleviating HM stress in P. pubescens, which warrants further study.
Keywords: Mn hyperaccumulator; Passivation; Phosphate fertilization; Phytoremediation efficiency.
Copyright © 2020 Elsevier Ltd. All rights reserved.