Rapid detection of Enterobacterales that produce carbapenemases

Diagn Microbiol Infect Dis. 2020 Oct;98(2):115120. doi: 10.1016/j.diagmicrobio.2020.115120. Epub 2020 Jun 26.

Abstract

Purpose: The rapid detection of carbapenemases among Enterobacterales in clinical laboratories is critical for management of patients, and infection prevention and control efforts.

Methods: A study was designed to evaluate the performances of the RAPIDEC CARBA NP®, β-CARBA®, NG-Test CARBA 5®, modified carbapenem-inactivation method, and EDTA version (eCIM) assays against a global collection of Enterobacterales (n = 216) with diverse carbapenemases.

Results: The RAPIDEC CARBA NP® assay had a sensitivity of 98.6% and specificity of 19.6% and β-CARBA® a sensitivity of 94% and specificity of 97.8%, but showed low sensitivity with Klebsiella Pneumoniae Carbapenemase (KPC)-containing isolates. The NG-Test CARBA 5® had an overall sensitivity of 96.3% and specificity of 100% and failed to detect isolates with blaIMP-13, blaIMP-14. The eCIM gave false- positive results with Oxacillinase (OXA)-48-like enzymes.

Conclusion: The NG-Test CARBA 5® assay was technically simple and provided rapid accurate results on the types of carbapenemases. Such information has potential treatment benefits for patients.

Keywords: Carbapenemases; Enterobacterales; Laboratory detection; Rapid.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / analysis*
  • Bacteriological Techniques / methods*
  • Drug Resistance, Multiple, Bacterial
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / diagnosis
  • Enterobacteriaceae Infections / microbiology
  • Humans
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • beta-Lactamases / analysis*

Substances

  • Bacterial Proteins
  • Reagent Kits, Diagnostic
  • beta-Lactamases
  • carbapenemase