Acclimatization of anaerobic sludge with cow manure and realization of high-rate food waste digestion for biogas production

Bao-Shan Xing; Yule Han; Xiaochang C Wang; Sifan Cao; Junwei Wen; Kaidi Zhang

doi:10.1016/j.biortech.2020.123830

Acclimatization of anaerobic sludge with cow manure and realization of high-rate food waste digestion for biogas production

Bioresour Technol. 2020 Nov:315:123830. doi: 10.1016/j.biortech.2020.123830. Epub 2020 Jul 13.

Authors

Bao-Shan Xing¹, Yule Han¹, Xiaochang C Wang², Sifan Cao¹, Junwei Wen¹, Kaidi Zhang¹

Affiliations

¹ International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi, China; Key Laboratory of Environmental Engineering, Shaanxi, China; Xi'an University of Architecture and Technology, No., 13 Yanta Road, Xi'an 710055, China.
² International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi, China; Key Laboratory of Environmental Engineering, Shaanxi, China; Xi'an University of Architecture and Technology, No., 13 Yanta Road, Xi'an 710055, China. Electronic address: xcwang@xauat.edu.cn.

PMID: 32688256
DOI: 10.1016/j.biortech.2020.123830

Abstract

Long-term acclimatization of anaerobic sludge was conducted by operating a mesophilic continuously stirred anaerobic reactor (CSTR) with continuous feeding of food wastes (FW) and cow manure (CM). During the long-term acclimatization, continued increase of enzyme activity was revealed, while the microbial structure tended stable as shown by the Shannon index and microbial community. By biomethane potential analysis, the acclimated sludge had a methane yield about 13 times higher than the initial anaerobic sludge. The acclimated sludge was subsequently used for FW digestion with stepwise organic loading rate increase without CM addition. The functional phyla of Bacteroidetes and Proteobacteria, which originated from CM but not very abundant, were significantly enriched not only during sludge acclimatization with CM addition but also in the process of FW digestion without CM addition. A microbe coexistence network was proposed to support an explanation of the metabolic pathways of FW digestion using the acclimated sludge.

Keywords: Acclimatization; Anaerobic digestion; Biogas; CSTR; Cow manure; Food waste.

MeSH terms

Acclimatization
Anaerobiosis
Animals
Biofuels*
Bioreactors
Cattle
Female
Food
Manure
Methane
Refuse Disposal*
Sewage

Substances

Biofuels
Manure
Sewage
Methane