Abstract
Roots grow asymmetrically, sometimes helically, around their growth direction likely to facilitate environmental sensing. We recently demonstrated that nitrate deficiency induces root coiling on horizontal surface through nitrate transporter/sensor NRT1.1 and PIN2- and AUX-mediated polar auxin transport. Here, we show that nitrate deficiency or NRT1.1 loss-of-function induces differential distribution of PIN2 between the future concave and concave sides in root epidermal cells. Treatment with pharmacological drugs suggests that enhanced endocytosis at the future convex side leads to reduced plasma membrane (PM) association of PIN2. A reduction of PIN2 at the PM would maintain a low auxin response to further enhance endocytosis at the convex side, leading to root coiling.
Keywords:
BFA; Nitrate; asymmetric root growth; auxin; endocytosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anion Transport Proteins / genetics
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Anion Transport Proteins / metabolism*
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Endocytosis / genetics
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Endocytosis / physiology*
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Gene Expression Regulation, Plant / genetics
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Gene Expression Regulation, Plant / physiology
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Indoleacetic Acids / metabolism
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Nitrates / metabolism*
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plant Roots / genetics
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Plant Roots / metabolism*
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Signal Transduction / genetics
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Signal Transduction / physiology
Substances
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Anion Transport Proteins
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Arabidopsis Proteins
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Indoleacetic Acids
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NRT1.1 protein, Arabidopsis
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Nitrates
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Plant Proteins
Grants and funding
This work was supported by the National Natural Science Foundation of China [31970332]; National Natural Science Foundation of China [31771558].