Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Felipe Amstalden Trevisan; Andressa Romualdo Rodrigues; Fermino Sanches Lizarte Neto; Fernanda Maris Peria; Múcio Luiz de Assis Cirino; Daniela Pretti da Cunha Tirapelli; Carlos Gilberto Carlotti Júnior

doi:10.1016/j.rpor.2020.06.007

Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Rep Pract Oncol Radiother. 2020 Sep-Oct;25(5):714-719. doi: 10.1016/j.rpor.2020.06.007. Epub 2020 Jul 10.

Authors

Felipe Amstalden Trevisan¹, Andressa Romualdo Rodrigues², Fermino Sanches Lizarte Neto¹, Fernanda Maris Peria³, Múcio Luiz de Assis Cirino¹, Daniela Pretti da Cunha Tirapelli¹, Carlos Gilberto Carlotti Júnior¹

Affiliations

¹ Medical School of Ribeirão Preto, University of São Paulo (USP), Department of Surgery and Anatomy, 3900 Bandeirantes Avenue, Ribeirão Preto, 14049-900, Brazil.
² Medical School of Franca, University of Franca (UNIFRAN), 201 Armando Salles Oliveira Avenue, Franca, 14404-600, Brazil.
³ Medical School of Ribeirão Preto, University of São Paulo (USP), Department of Internal Medicine, 3900 Bandeirantes Avenue, Ribeirão Preto, 14049-900, Brazil.

Abstract

Aim: To evaluate the effect of radiotherapy and temozolomide on the expression of miRNAs apoptotic (miRNAs-21, -221, -222 (anti-apoptotic) and miRNAs-15a, -16 (pro-apoptotic)) and the gene MGMT in glioblastoma cell lines.

Background: The limited knowledge of the molecular biology of malignant gliomas may hinder the development of therapeutic modalities. In this scenario, one of the greatest advances of recent years was the identification of microRNAs. These molecules have an important role in biological processes involving cancer, including glioblastoma.

Materials and methods: Trypan blue was used to verify the cell viability, and real time PCR to quantify the expression of microRNAs and gene 24, 48 and 120 h after exposure to treatments.

Results: There was a statistically significant decrease of expression of miR-15a between 48 and 120 h in line T98 G treated with radiation, increased expression of miR-15a between 24 and 120 h in line U251 treated with radiation and temozolomide, and increased expression of miR-16 between 24 and 120 h in line U251 treated with radiation alone and when combined with temozolomide. There was a decrease in MGMT gene expression, between 24 and 48 h in U343 cells treated with temozolomide.

Conclusions: Ionizing radiation and temozolomide modified the expression of miRNAs studied and MGMT.

Keywords: Apoptosis; Glioblastoma; Ionizing radiation; microRNAs.