Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States

Bidisha Chanda; Yazmín Rivera; Schyler O Nunziata; Marco E Galvez; Andrea Gilliard; Kai-Shu Ling

doi:10.1128/MRA.00630-20

Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States

Microbiol Resour Announc. 2020 Jul 16;9(29):e00630-20. doi: 10.1128/MRA.00630-20.

Authors

Bidisha Chanda¹, Yazmín Rivera², Schyler O Nunziata², Marco E Galvez², Andrea Gilliard¹, Kai-Shu Ling³

Affiliations

¹ USDA-Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA.
² USDA-APHIS-PPQ Center for Plant Health Science and Technology, Beltsville, Maryland, USA.
³ USDA-Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA kai.ling@usda.gov.

Abstract

The complete genome sequence of a U.S. isolate of a Tomato brown rugose fruit virus (ToBRFV) (CA18-01) was obtained through Illumina and MinION sequencing. The U.S. ToBRFV isolate shared a high nucleic acid sequence identity (>99%) with known ToBRFV isolates. Phylogenetic analysis revealed a tight cluster for ToBRFV isolates throughout the world, suggesting a short evolutionary history.