Different responses after intracerebral hemorrhage between young and early middle-aged mice

Keita Kinoshita; Ryo Ohtomo; Hajime Takase; Gen Hamanaka; Kelly K Chung; Josephine Lok; Hiroshi Katsuki; Ken Arai

doi:10.1016/j.neulet.2020.135249

Different responses after intracerebral hemorrhage between young and early middle-aged mice

Neurosci Lett. 2020 Sep 14:735:135249. doi: 10.1016/j.neulet.2020.135249. Epub 2020 Jul 13.

Authors

Keita Kinoshita¹, Ryo Ohtomo², Hajime Takase³, Gen Hamanaka⁴, Kelly K Chung⁴, Josephine Lok⁴, Hiroshi Katsuki⁵, Ken Arai⁶

Affiliations

¹ Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA; Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
² Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA; Department of Neurology, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.
³ Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA; Department of Neurosurgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
⁴ Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
⁵ Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
⁶ Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA. Electronic address: karai@partners.org.

Abstract

Although aging is a major risk factor for intracerebral hemorrhage (ICH), there are very few studies comparing ICH pathology between young and early middle-aged mice. In this study, 8-month old mice (early middle-aged mice) were compared against 2-month old mice (young mice) in neurological and histological changes after ICH induction, such as body weight, lesion volume, astrocytic responses, and motor and cognitive functions. At day 8 after ICH, there was no significant difference in lesion volume between the two groups, and both groups did not exhibit significant cognitive decline, as assessed by spontaneous alternative Y-maze test. On the other hand, 8-month old mice showed delayed recovery from body weight loss, along with reduced astrocytic activation. Interestingly, in the two motor function tests (beam-walking test and corner turn test), 8-month old mice exhibited lower scores only in the beam-walking test, suggesting a partial disturbance in motor recovery after ICH. These results suggest that age-related differences in ICH pathology may already start to appear in early middle-aged brains.

Keywords: Astrocytes; Intracerebral hemorrhage; Middle-aged mice; Motor dysfunction.

Publication types

Comparative Study
Research Support, N.I.H., Extramural

MeSH terms

Aging / metabolism*
Aging / pathology
Animals
Cerebral Hemorrhage / metabolism*
Cerebral Hemorrhage / pathology
Cognition / physiology*
Male
Maze Learning / physiology*
Mice
Mice, Inbred C57BL
Motor Activity / physiology*
Recovery of Function / physiology*

Abstract

Publication types

MeSH terms

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