Integrated Analysis of DEAD-Box Helicase 56: A Potential Oncogene in Osteosarcoma

Chen Zhu; Xianzuo Zhang; Nikolaos Kourkoumelis; Yong Shen; Wei Huang

doi:10.3389/fbioe.2020.00588

Integrated Analysis of DEAD-Box Helicase 56: A Potential Oncogene in Osteosarcoma

Front Bioeng Biotechnol. 2020 Jun 26:8:588. doi: 10.3389/fbioe.2020.00588. eCollection 2020.

Authors

Chen Zhu¹, Xianzuo Zhang¹, Nikolaos Kourkoumelis², Yong Shen^{3

4}, Wei Huang¹

Affiliations

¹ Division of Life Sciences and Medicine, Department of Orthopedics, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China.
² Department of Medical Physics, School of Health Sciences, University of Ioannina, Ioannina, Greece.
³ Institute on Aging and Brain Disorders, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China.
⁴ Division of Life Sciences and Medicine, Neurodegenerative Disorder Research Center, University of Science and Technology of China, Hefei, China.

Abstract

Background: Osteosarcoma is a solid tumor common in the musculoskeletal system. The DEAD-box helicase (DDX) families play an important role in tumor genesis and proliferation. Objective:To screen potential molecular targets in osteosarcoma and elucidate its relationship with DDX56. Methods: We employed the Gene Expression Omnibus and The Cancer Genome Atlas datasets for preliminary screening. DDX56 expression was measured by RT-qPCR in three osteosarcoma cell lines. Biological roles of DDX56 were explored by Gene ontology, Kyoto Encyclopedia of Genes and Genomes and Ingenuity Pathway Analysis. Cell proliferation, cycle, and apoptosis assays were performed using Lentivirus™ knockdown technique. Results: It was found that DDX56 expression was regularly upregulated in osteosarcoma tissue and cell lines, while DDX56 knockdown inhibited cell proliferation and promoted cell apoptosis. Conclusions: The findings suggest DDX56 as a potential therapeutic target for the treatment of osteosarcoma.

Keywords: DEAD-box RNA helicases 56 (DDX56); biomarker; oncogene; osteosarcoma; proliferation.