Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp

Yuan-Yuan Zang; Sha Yang; Yong-Qiang Xu; Zhi-Gang Chen; Tao Wu

doi:10.3389/fbioe.2020.00470

Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp

Front Bioeng Biotechnol. 2020 Jun 26:8:470. doi: 10.3389/fbioe.2020.00470. eCollection 2020.

Authors

Yuan-Yuan Zang¹, Sha Yang¹, Yong-Qiang Xu², Zhi-Gang Chen¹, Tao Wu³

Affiliations

¹ Glycomics and Glycan Bioengineering Research Center, College of Food Science & Technology, Nanjing Agricultural University, Nanjing, China.
² College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
³ Department of Food Science, University of Tennessee, Knoxville, Knoxville, TN, United States.

Abstract

In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 - 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40^oC with the degradation rate of 10.65 mg min^-1 L^-1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production.

Keywords: cross-linked enzyme aggregate (CLEA); enzymatic characteristics; fusarium; natural deep eutectic solvents (NADESs); rutin degrading enzyme (RDE).