Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology

Santhana Krishnan; B Noor Suzana; Zularisam Abdul Wahid; Mohd Nasrullah; Mimi Sakinah Abdul Munaim; Mohd Fadhil Bin Md Din; Shazwin Mat Taib; Yu You Li

doi:10.1016/j.btre.2020.e00498

Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology

Biotechnol Rep (Amst). 2020 Jun 29:27:e00498. doi: 10.1016/j.btre.2020.e00498. eCollection 2020 Sep.

Authors

Santhana Krishnan¹, B Noor Suzana², Zularisam Abdul Wahid³, Mohd Nasrullah³, Mimi Sakinah Abdul Munaim^{2

3}, Mohd Fadhil Bin Md Din¹, Shazwin Mat Taib⁴, Yu You Li⁵

Affiliations

¹ Center of Environmental Sustainability and Water Security (IPASA), Research Institute of Sustainable Environment (RISE), School of Civil Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
² Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Kuantan Pahang, Malaysia.
³ Faculty of Civil Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Kuantan, Pahang, Malaysia.
⁴ Center for Coastal and Ocean Engineering, Research Institute for Sustainable Environment, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia.
⁵ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, 980-8579, Japan.

Abstract

The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0-100), transmembrane pressure (TMP) (1-1.6 bar), cross flow velocity (CFV) (0.52-1.2 cm/s^-1) and its combined effect to obtain high flux with less possibility of membrane fouling. Experimental studies revealed that the best range for optimization process for filtration time, operational transmembrane pressure and cross flow velocity was 30 min, 1.4 bars and 1.06 cm/s, respectively as these conditions yielded the highest membrane permeability (56.03 Lm^-2h-¹ bar^-1) and xylitol content (15.49 g/l). According to the analysis of variance (ANOVA), the p-value (<0.0001) indicated the designed model was highly significant. The error percentage between the actual and predicted value for membrane permeability and xylitol amount (2.21 % and 4.85 % respectively), which both were found to be close to the predicted values. The verification experiments gave membrane actual permeability of 57.3 Lm^-2h-¹ bar^-1 and 16.29 g/l of xylitol production, thus indicating that the successfully developed model to predict the response.

Keywords: Central composite design (CCD); Cross flow technique; Ultrafiltration (UF); Xylose reductase (XR).