Direct on-the-spot detection of SARS-CoV-2 in patients

Exp Biol Med (Maywood). 2020 Aug;245(14):1187-1193. doi: 10.1177/1535370220941819. Epub 2020 Jul 16.

Abstract

Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.

Keywords: Covid-19; Molecular; RT-LAMP; SARS-CoV-2; pandemic; surveillance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Betacoronavirus / isolation & purification
  • COVID-19
  • Coronavirus Infections / diagnosis*
  • Humans
  • Mass Screening / methods*
  • Molecular Diagnostic Techniques / methods
  • Nucleic Acid Amplification Techniques / methods
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • SARS-CoV-2
  • Saliva / virology
  • Sensitivity and Specificity

Supplementary concepts

  • LAMP assay