The large-scale and label-free molecular characterization of single cells in their natural tissue habitat remains a major challenge in molecular biology. We present a method that integrates morphometric image analysis to delineate and classify individual cells with their single-cell-specific molecular profiles. This approach provides a new means to study spatial biological processes such as cancer field effects and the relationship between morphometric and molecular features.
Keywords: imaging; lipids; mass spectrometry; morphometry; single-cell analysis.
© 2020 The Authors. Published by Wiley-VCH GmbH.