Abstract
We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.
MeSH terms
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Animals
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Fibroblasts / immunology
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Fibroblasts / transplantation
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Genetic Engineering*
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Genetic Vectors
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HeLa Cells / immunology
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HeLa Cells / pathology
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Humans
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Interleukin-2 / biosynthesis
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Interleukin-2 / genetics*
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Killer Cells, Natural / immunology
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Mice
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Mice, Nude
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Neoplasm Transplantation
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Neoplasms, Experimental / genetics
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Neoplasms, Experimental / immunology
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Neoplasms, Experimental / therapy*
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Retroviridae / genetics
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Transformation, Genetic