2-Methylfuran: Toxicity and genotoxicity in male Sprague-Dawley rats

Jiao Huo; Yunjie Liu; Xuejiao Zhu; Zhu Zeng; Yiyi Chen; Ruirui Li; Lishi Zhang; Jinyao Chen

doi:10.1016/j.mrgentox.2020.503209

2-Methylfuran: Toxicity and genotoxicity in male Sprague-Dawley rats

Mutat Res Genet Toxicol Environ Mutagen. 2020 Jun-Jul:854-855:503209. doi: 10.1016/j.mrgentox.2020.503209. Epub 2020 Jun 15.

Authors

Jiao Huo¹, Yunjie Liu², Xuejiao Zhu³, Zhu Zeng³, Yiyi Chen³, Ruirui Li³, Lishi Zhang³, Jinyao Chen⁴

Affiliations

¹ Department of Nutrition, Food Safety and Toxicology, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China; Chongqing Center for Disease Control and Prevention, Chongqing, China.
² Graduate Department, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China.
³ Department of Nutrition, Food Safety and Toxicology, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China; Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, Sichuan, China.
⁴ Department of Nutrition, Food Safety and Toxicology, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China; Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, Sichuan, China. Electronic address: umbrellayy@163.com.

PMID: 32660823
DOI: 10.1016/j.mrgentox.2020.503209

Abstract

We have measured the toxicity and genotoxicity of 2-methylfuran, which is formed in foods during thermal processing. The agent was administered by oral gavage to male Sprague-Dawley rats, daily for 28 days, before performing general toxicology analysis and the following genotoxicity tests: comet assay (peripheral blood, liver); Pig-a gene mutation assay (peripheral blood); micronucleus test (peripheral blood, bone marrow). Liver was the primary target organ; histological changes (oval cell hyperplasia) were observed but without significant changes in serum enzyme markers. For hepatotoxicity, the no-observed-adverse-effect level was 5 mg/kg bw/d. Histopathological changes were also seen in the bone marrow. Genotoxicity assays were uniformly negative.

Keywords: 2-Methylfuran; Micronucleus; Multiendpoint; NOAEL; Pig-a.

MeSH terms

Animals
Comet Assay
DNA Damage / drug effects
Furans / toxicity*
Liver / drug effects
Male
Micronucleus Tests / methods
Mutagenicity Tests / methods
Mutagens / toxicity
Rats
Rats, Sprague-Dawley

Substances

Furans
Mutagens
2-methylfuran