Proteomic modelling of gluten digestion from a physiologically relevant food system: A focus on the digestion of immunogenic peptides from wheat implicated in celiac disease

Olivia Ogilvie; Sarah Roberts; Kevin Sutton; Laura Domigan; Nigel Larsen; Juliet Gerrard; Nicholas Demarais

doi:10.1016/j.foodchem.2020.127466

Proteomic modelling of gluten digestion from a physiologically relevant food system: A focus on the digestion of immunogenic peptides from wheat implicated in celiac disease

Food Chem. 2020 Dec 15:333:127466. doi: 10.1016/j.foodchem.2020.127466. Epub 2020 Jul 5.

Authors

Olivia Ogilvie¹, Sarah Roberts², Kevin Sutton³, Laura Domigan⁴, Nigel Larsen⁵, Juliet Gerrard⁶, Nicholas Demarais⁷

Affiliations

¹ School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; The New Zealand Institute for Plant and Food Research Limited, Private Bag 4704, Christchurch Mail Centre, Christchurch 8140, New Zealand. Electronic address: oogi280@aucklanduni.ac.nz.
² Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; The New Zealand Institute for Plant and Food Research Limited, Private Bag 4704, Christchurch Mail Centre, Christchurch 8140, New Zealand. Electronic address: sarah.roberts@plantandfood.co.nz.
³ Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; The New Zealand Institute for Plant and Food Research Limited, Private Bag 4704, Christchurch Mail Centre, Christchurch 8140, New Zealand. Electronic address: kevin.sutton@plantandfood.co.nz.
⁴ School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; Department of Chemical and Materials Engineering The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: l.domigan@auckland.ac.nz.
⁵ Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; The New Zealand Institute for Plant and Food Research Limited, Private Bag 4704, Christchurch Mail Centre, Christchurch 8140, New Zealand.
⁶ School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: j.gerrard@auckland.ac.nz.
⁷ School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Department of Chemical and Materials Engineering The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: n.demarais@auckland.ac.nz.

PMID: 32659668
DOI: 10.1016/j.foodchem.2020.127466

Abstract

Celiac disease is an autoimmune illness activated by gluten peptides produced during gastrointestinal digestion. A simulated in vitro digestion of gluten was conducted to define the profile and kinetic release pattern of immunogenic gluten peptides in a physiologically relevant food matrix. White bread was digested using the INFOGEST in vitro standardised digestion protocol from 0 to 240 min and subsequently analysed by SDS-PAGE, quantitative LC-MS/MS, untargeted LC-MS/MS and ELISA. The release profile of six gluten peptides was defined by quantitative LC-MS/MS; none were detected in the gastric phase, but rapidly peaked in the intestinal phase. These results were corroborated by the ELISA analysis. Untargeted proteomics identified 83 immunogenic peptides. Their qualitative concentrations were defined throughout digestion, demonstrating complex relationships through proteolysis. This analysis suggests immunogenic gluten may peak within the intestinal duodenum and gives new insights into the complexity of gluten digestion from a physiologically relevant food matrix.

Keywords: Bread; Celiac disease; Digestion; Gluten; Mass spectrometry; Peptidomics; Proteomics.

MeSH terms

Bread / analysis*
Celiac Disease / immunology*
Chromatography, Liquid
Digestion
Glutens / analysis
Glutens / immunology
Glutens / metabolism*
Humans
Peptides / analysis*
Proteomics*
Tandem Mass Spectrometry
Triticum / chemistry

Substances

Peptides
Glutens