This Healthcare Cost and Utilization Project (HCUP) Statistical Brief focuses on the hospital burden of opioid-related hospitalizations by hospital urban-rural location. The Statistical Brief presents hospital statistics on opioid-related inpatient stays using the 2016 State Inpatient Databases (SID) from 46 States and the District of Columbia. The hospital rate of opioid-related stays per 1,000 inpatient stays is examined for a total of 4,207 community hospitals (excluding rehabilitation and long-term acute care hospitals) classified based on the urban-rural location of the hospital: metropolitan (metro), rural and adjacent to a metropolitan area (rural-adjacent), and rural and not adjacent to a metropolitan area (rural-remote). The hospital rate of opioid-related stays is presented by hospital urban-rural location for each U.S. census division. The distribution of the rate is provided across all metropolitan and rural hospitals.