The genome of the butternut canker pathogen, Ophiognomonia clavigignenti-juglandacearum shows an elevated number of genes associated with secondary metabolism and protection from host resistance responses

Guangxi Wu; Taruna A Schuelke; Gloria Iriarte; Kirk Broders

doi:10.7717/peerj.9265

The genome of the butternut canker pathogen, Ophiognomonia clavigignenti-juglandacearum shows an elevated number of genes associated with secondary metabolism and protection from host resistance responses

PeerJ. 2020 Jun 29:8:e9265. doi: 10.7717/peerj.9265. eCollection 2020.

Authors

Guangxi Wu¹, Taruna A Schuelke², Gloria Iriarte³, Kirk Broders³

Affiliations

¹ Department of Agricultural Biology, Colorado State University, Fort Collins, CO, USA.
² Ecology, Evolution and Marine Biology Department, University of California, Santa Barbara, Santa Barbara, CA, USA.
³ Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama.

Abstract

Ophiognomonia clavigignenti-juglandacearum (Oc-j) is a plant pathogenic fungus that causes canker and branch dieback diseases in the hardwood tree butternut, Juglans cinerea. Oc-j is a member of the order of Diaporthales, which includes many other plant pathogenic species, several of which also infect hardwood tree species. In this study, we sequenced the genome of Oc-j and achieved a high-quality assembly and delineated its phylogeny within the Diaporthales order using a genome-wide multi-gene approach. We also further examined multiple gene families that might be involved in plant pathogenicity and degradation of complex biomass, which are relevant to a pathogenic life-style in a tree host. We found that the Oc-j genome contains a greater number of genes in these gene families compared to other species in the Diaporthales. These gene families include secreted CAZymes, kinases, cytochrome P450, efflux pumps, and secondary metabolism gene clusters. The large numbers of these genes provide Oc-j with an arsenal to cope with the specific ecological niche as a pathogen of the butternut tree.

Keywords: Canker pathogen; Cytochrome P450; Diaporthales; Efflux pump; Fagales; Juglans; Pathogen evolution.

Grants and funding

Funding from this project was provided from grant number 14NB49 from the National Geographic Society. Dr. Broders was also supported by the Simon’s Foundation Grant number 429440 to the Smithsonian Tropical Research Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.