Long noncoding RNA MALAT1 regulates apoptosis in ischemic stroke by sponging miR-205-3p and modulating PTEN expression

Qi Gao; Yanfeng Wang

Long noncoding RNA MALAT1 regulates apoptosis in ischemic stroke by sponging miR-205-3p and modulating PTEN expression

Am J Transl Res. 2020 Jun 15;12(6):2738-2748. eCollection 2020.

Authors

Qi Gao¹, Yanfeng Wang¹

Affiliation

¹ Department of Neurology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University Huai'an 223300, Jiangsu, China.

PMID: 32655805
PMCID: PMC7344059

Abstract

Ischemic stroke has been considered to be one of the major causes of disability worldwide which related to multiple pathological processes including apoptosis. Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), is one of the long non-coding RNA (lncRNA) know as a regulator for cell apoptosis. However, further and deeper cellular and molecular mechanism in stroke model remains unclear. The results showed MALAT1 was down-regulated in OGD-induced apoptosis and related with miR-205-3p expression. Knockdown of MALAT1 promote OGD-induced apoptosis, decreased the cell viability, inhibit the caspase-3 activation. Moreover, MALAT1 acts as a competing endogenous RNA (ceRNA) for miR-205-3p and further regulate PTEN expression protect OGD-induced apoptosis. Altogether, these results suggest that MALAT1 may suppress the apoptosis in ischemic stroke and function as a ceRNA for miR-205-3p to modulate PTEN expression. These findings may provide a novel therapeutic target for treating ischemic stroke.

Keywords: LncRNA; MALAT1; PTEN; apoptosis; competing endogenous RNA; endothelial cells; ischemic stroke; miR-205-3p.